How to find the amino acid in the DNA protein

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 3' A T A G T A C C G C A T G T A C G G G C G A G A C A T T C G A G C A T T C A T 5'


This a Template DNA.



How to find the number of amino acids amino acids in the protein encoded by the above gene?



The answer is $7$.



My Try:



First I converted the above DNA to RNA and got



 5' U A U C A U G G C G U A C A U G C C C G C U C U G U A A G C U C G U A A G U A 3'


Then I found the start codon which is A U G



5' U A U C |A U G| G C G U A C A U G C C C G C U C U G U A A G C U C G U A A G U A 3'


From here I am not understanding how to proceed.



Can anyone please explain how to solve this?







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  • Short question: How does RNA translate into protein?
    – Chris♦
    Aug 5 at 19:34














up vote
2
down vote

favorite












 3' A T A G T A C C G C A T G T A C G G G C G A G A C A T T C G A G C A T T C A T 5'


This a Template DNA.



How to find the number of amino acids amino acids in the protein encoded by the above gene?



The answer is $7$.



My Try:



First I converted the above DNA to RNA and got



 5' U A U C A U G G C G U A C A U G C C C G C U C U G U A A G C U C G U A A G U A 3'


Then I found the start codon which is A U G



5' U A U C |A U G| G C G U A C A U G C C C G C U C U G U A A G C U C G U A A G U A 3'


From here I am not understanding how to proceed.



Can anyone please explain how to solve this?







share|improve this question



















  • Short question: How does RNA translate into protein?
    – Chris♦
    Aug 5 at 19:34












up vote
2
down vote

favorite









up vote
2
down vote

favorite











 3' A T A G T A C C G C A T G T A C G G G C G A G A C A T T C G A G C A T T C A T 5'


This a Template DNA.



How to find the number of amino acids amino acids in the protein encoded by the above gene?



The answer is $7$.



My Try:



First I converted the above DNA to RNA and got



 5' U A U C A U G G C G U A C A U G C C C G C U C U G U A A G C U C G U A A G U A 3'


Then I found the start codon which is A U G



5' U A U C |A U G| G C G U A C A U G C C C G C U C U G U A A G C U C G U A A G U A 3'


From here I am not understanding how to proceed.



Can anyone please explain how to solve this?







share|improve this question











 3' A T A G T A C C G C A T G T A C G G G C G A G A C A T T C G A G C A T T C A T 5'


This a Template DNA.



How to find the number of amino acids amino acids in the protein encoded by the above gene?



The answer is $7$.



My Try:



First I converted the above DNA to RNA and got



 5' U A U C A U G G C G U A C A U G C C C G C U C U G U A A G C U C G U A A G U A 3'


Then I found the start codon which is A U G



5' U A U C |A U G| G C G U A C A U G C C C G C U C U G U A A G C U C G U A A G U A 3'


From here I am not understanding how to proceed.



Can anyone please explain how to solve this?









share|improve this question










share|improve this question




share|improve this question









asked Aug 5 at 19:31









philip

404




404











  • Short question: How does RNA translate into protein?
    – Chris♦
    Aug 5 at 19:34
















  • Short question: How does RNA translate into protein?
    – Chris♦
    Aug 5 at 19:34















Short question: How does RNA translate into protein?
– Chris♦
Aug 5 at 19:34




Short question: How does RNA translate into protein?
– Chris♦
Aug 5 at 19:34










2 Answers
2






active

oldest

votes

















up vote
3
down vote



accepted










Then you just have to read the codon until you reach a stop codon. There are three stop codon UAA, UGA and UAG. So, in your example..



 Start Stop
5' U A U C | A U G | G C G | U A C | A U G | C C C | G C U | C U G | U A A | G C U C G U A A G U A 3'


Your protein is therefore 7 amino acids long (incuding the starting methionine). The genetic code is
enter image description here



Therefore the 7 amino acids are



Met Ala Tyr Met Pro Ala Leu


In case you are confused about having an AUG codon in the middle of an open reading frame, then you should have a look at the post Effect of a doubling of the start codon in a gene.



Of course, I assumed that the region is indeed transcribed and that the first AUG is indeed the start codon and not just a methionine in the middle of an open reading frame.






share|improve this answer



















  • 1




    Good answer, though perhaps you should include the first methionine as the N-terminus since it is not always cleaved and the answer given in the OP is 7.
    – canadianer
    2 days ago










  • @canadianer Thank you! Edited. I did not know the methionine of the start codon was sometimes (often, usually?!) kept.
    – Remi.b
    2 days ago

















up vote
0
down vote













Each protein-coding gene consists of coding and non-coding regions. Coding region, a.k.a CDS (see: Coding region and Open Reading Frame), is spanned between translation initiation site (TIS), and one of stop codons. Non-coding regions include introns and non translated regions (5'UTR or 3'UTR) in the exons.



You need to know first, if (and where) your DNA template contains the information about amino acids sequence. Does your DNA template is the protein-coding region only or do you have some non-coding parts (eg. introns).



(Short remark: Methionine can be also found within the amino acids chain, not only at TIS = not every methionine is a TIS)






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    2 Answers
    2






    active

    oldest

    votes








    2 Answers
    2






    active

    oldest

    votes









    active

    oldest

    votes






    active

    oldest

    votes








    up vote
    3
    down vote



    accepted










    Then you just have to read the codon until you reach a stop codon. There are three stop codon UAA, UGA and UAG. So, in your example..



     Start Stop
    5' U A U C | A U G | G C G | U A C | A U G | C C C | G C U | C U G | U A A | G C U C G U A A G U A 3'


    Your protein is therefore 7 amino acids long (incuding the starting methionine). The genetic code is
    enter image description here



    Therefore the 7 amino acids are



    Met Ala Tyr Met Pro Ala Leu


    In case you are confused about having an AUG codon in the middle of an open reading frame, then you should have a look at the post Effect of a doubling of the start codon in a gene.



    Of course, I assumed that the region is indeed transcribed and that the first AUG is indeed the start codon and not just a methionine in the middle of an open reading frame.






    share|improve this answer



















    • 1




      Good answer, though perhaps you should include the first methionine as the N-terminus since it is not always cleaved and the answer given in the OP is 7.
      – canadianer
      2 days ago










    • @canadianer Thank you! Edited. I did not know the methionine of the start codon was sometimes (often, usually?!) kept.
      – Remi.b
      2 days ago














    up vote
    3
    down vote



    accepted










    Then you just have to read the codon until you reach a stop codon. There are three stop codon UAA, UGA and UAG. So, in your example..



     Start Stop
    5' U A U C | A U G | G C G | U A C | A U G | C C C | G C U | C U G | U A A | G C U C G U A A G U A 3'


    Your protein is therefore 7 amino acids long (incuding the starting methionine). The genetic code is
    enter image description here



    Therefore the 7 amino acids are



    Met Ala Tyr Met Pro Ala Leu


    In case you are confused about having an AUG codon in the middle of an open reading frame, then you should have a look at the post Effect of a doubling of the start codon in a gene.



    Of course, I assumed that the region is indeed transcribed and that the first AUG is indeed the start codon and not just a methionine in the middle of an open reading frame.






    share|improve this answer



















    • 1




      Good answer, though perhaps you should include the first methionine as the N-terminus since it is not always cleaved and the answer given in the OP is 7.
      – canadianer
      2 days ago










    • @canadianer Thank you! Edited. I did not know the methionine of the start codon was sometimes (often, usually?!) kept.
      – Remi.b
      2 days ago












    up vote
    3
    down vote



    accepted







    up vote
    3
    down vote



    accepted






    Then you just have to read the codon until you reach a stop codon. There are three stop codon UAA, UGA and UAG. So, in your example..



     Start Stop
    5' U A U C | A U G | G C G | U A C | A U G | C C C | G C U | C U G | U A A | G C U C G U A A G U A 3'


    Your protein is therefore 7 amino acids long (incuding the starting methionine). The genetic code is
    enter image description here



    Therefore the 7 amino acids are



    Met Ala Tyr Met Pro Ala Leu


    In case you are confused about having an AUG codon in the middle of an open reading frame, then you should have a look at the post Effect of a doubling of the start codon in a gene.



    Of course, I assumed that the region is indeed transcribed and that the first AUG is indeed the start codon and not just a methionine in the middle of an open reading frame.






    share|improve this answer















    Then you just have to read the codon until you reach a stop codon. There are three stop codon UAA, UGA and UAG. So, in your example..



     Start Stop
    5' U A U C | A U G | G C G | U A C | A U G | C C C | G C U | C U G | U A A | G C U C G U A A G U A 3'


    Your protein is therefore 7 amino acids long (incuding the starting methionine). The genetic code is
    enter image description here



    Therefore the 7 amino acids are



    Met Ala Tyr Met Pro Ala Leu


    In case you are confused about having an AUG codon in the middle of an open reading frame, then you should have a look at the post Effect of a doubling of the start codon in a gene.



    Of course, I assumed that the region is indeed transcribed and that the first AUG is indeed the start codon and not just a methionine in the middle of an open reading frame.







    share|improve this answer















    share|improve this answer



    share|improve this answer








    edited 2 days ago


























    answered 2 days ago









    Remi.b

    54k6100177




    54k6100177







    • 1




      Good answer, though perhaps you should include the first methionine as the N-terminus since it is not always cleaved and the answer given in the OP is 7.
      – canadianer
      2 days ago










    • @canadianer Thank you! Edited. I did not know the methionine of the start codon was sometimes (often, usually?!) kept.
      – Remi.b
      2 days ago












    • 1




      Good answer, though perhaps you should include the first methionine as the N-terminus since it is not always cleaved and the answer given in the OP is 7.
      – canadianer
      2 days ago










    • @canadianer Thank you! Edited. I did not know the methionine of the start codon was sometimes (often, usually?!) kept.
      – Remi.b
      2 days ago







    1




    1




    Good answer, though perhaps you should include the first methionine as the N-terminus since it is not always cleaved and the answer given in the OP is 7.
    – canadianer
    2 days ago




    Good answer, though perhaps you should include the first methionine as the N-terminus since it is not always cleaved and the answer given in the OP is 7.
    – canadianer
    2 days ago












    @canadianer Thank you! Edited. I did not know the methionine of the start codon was sometimes (often, usually?!) kept.
    – Remi.b
    2 days ago




    @canadianer Thank you! Edited. I did not know the methionine of the start codon was sometimes (often, usually?!) kept.
    – Remi.b
    2 days ago










    up vote
    0
    down vote













    Each protein-coding gene consists of coding and non-coding regions. Coding region, a.k.a CDS (see: Coding region and Open Reading Frame), is spanned between translation initiation site (TIS), and one of stop codons. Non-coding regions include introns and non translated regions (5'UTR or 3'UTR) in the exons.



    You need to know first, if (and where) your DNA template contains the information about amino acids sequence. Does your DNA template is the protein-coding region only or do you have some non-coding parts (eg. introns).



    (Short remark: Methionine can be also found within the amino acids chain, not only at TIS = not every methionine is a TIS)






    share|improve this answer

























      up vote
      0
      down vote













      Each protein-coding gene consists of coding and non-coding regions. Coding region, a.k.a CDS (see: Coding region and Open Reading Frame), is spanned between translation initiation site (TIS), and one of stop codons. Non-coding regions include introns and non translated regions (5'UTR or 3'UTR) in the exons.



      You need to know first, if (and where) your DNA template contains the information about amino acids sequence. Does your DNA template is the protein-coding region only or do you have some non-coding parts (eg. introns).



      (Short remark: Methionine can be also found within the amino acids chain, not only at TIS = not every methionine is a TIS)






      share|improve this answer























        up vote
        0
        down vote










        up vote
        0
        down vote









        Each protein-coding gene consists of coding and non-coding regions. Coding region, a.k.a CDS (see: Coding region and Open Reading Frame), is spanned between translation initiation site (TIS), and one of stop codons. Non-coding regions include introns and non translated regions (5'UTR or 3'UTR) in the exons.



        You need to know first, if (and where) your DNA template contains the information about amino acids sequence. Does your DNA template is the protein-coding region only or do you have some non-coding parts (eg. introns).



        (Short remark: Methionine can be also found within the amino acids chain, not only at TIS = not every methionine is a TIS)






        share|improve this answer













        Each protein-coding gene consists of coding and non-coding regions. Coding region, a.k.a CDS (see: Coding region and Open Reading Frame), is spanned between translation initiation site (TIS), and one of stop codons. Non-coding regions include introns and non translated regions (5'UTR or 3'UTR) in the exons.



        You need to know first, if (and where) your DNA template contains the information about amino acids sequence. Does your DNA template is the protein-coding region only or do you have some non-coding parts (eg. introns).



        (Short remark: Methionine can be also found within the amino acids chain, not only at TIS = not every methionine is a TIS)







        share|improve this answer













        share|improve this answer



        share|improve this answer











        answered 2 days ago









        hibernicah

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